ENCAB550TJZ
Antibody against Homo sapiens RPS3A
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- GeneTex
- Product ID
- GTX105020
- Lot ID
- 39953
- Characterized targets
- RPS3A (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
RPS3A (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using RPS3A specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-RPS3A antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- GeneTex_GTX105020_39953_RPS3A.png
RPS3A (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against RPS3A in K562 and HepG2 whole cell lysate using RPS3A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RPS3A. Lanes 5-8 follow the same pattern, but in HepG2. RPS3A protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- RPS3A_Secondary_Western.png