ENCAB529HWM

Antibody against Homo sapiens APOBEC3C

Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
GeneTex
Product ID
GTX102164
Lot ID
40968
Characterized targets
APOBEC3C (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

APOBEC3C (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against APOBEC3C in K562 and HepG2 whole cell lysate using APOBEC3C specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against APOBEC3C. Lanes 5-8 follow the same pattern, but in HepG2. APOBEC3C protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
APOBEC3C (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of APOBEC3C specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-APOBEC3C. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
APOBEC3C (Homo sapiens)
K562K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using APOBEC3C specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-APOBEC3C antibody (lanes under 'APOBEC3C'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD