ENCAB511RTZ
Antibody against Homo sapiens ARNT
Homo sapiens
K562
characterized to standards
Homo sapiens
GM12878, HepG2, liver
partially characterized
- Status
- released
- Source (vendor)
- Sigma
- Product ID
- HPA001759
- Lot ID
- A08754
- Characterized targets
- ARNT (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-766
- External resources
Characterizations
ARNT (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation of ARNT from K562 cells using HPA001759. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with HPA001759. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 87 kDa.
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1004_ARNT_HPA01759.jpg
ARNT (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA001759. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ARNT (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using HPA001759, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by Colloidial Coomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ARNT (Homo sapiens)
GM12878K562HepG2liver
not submitted for review by lab
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878,K562,HepG2,Liver using the antibody HPA001759. Molecular Weight: 86.636.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt 803_3-ARNT.jpg
- Documents