ENCAB511RTZ

Antibody against Homo sapiens ARNT

Homo sapiens
K562
characterized to standards
Homo sapiens
HepG2, liver, GM12878
partially characterized
Status
released
Source (vendor)
Sigma
Product ID
HPA001759
Lot ID
A08754
Characterized targets
ARNT (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-766
External resources

Characterizations

ARNT (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation of ARNT from K562 cells using HPA001759. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with HPA001759. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 87 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ARNT (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA001759. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ARNT (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using HPA001759, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by Colloidial Coomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ARNT (Homo sapiens)
GM12878K562HepG2liver
Method: immunoblot
Attachment from submitter
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878,K562,HepG2,Liver using the antibody HPA001759. Molecular Weight: 86.636.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford