1. Antibodies
  2. Homo sapiens
  3. HDAC1

ENCAB508CZJ

Antibody against Homo sapiens HDAC1

Homo sapiens
K562, MCF-7, GM12878
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Active Motif
Product ID
40967
Lot ID
04214009
Characterized targets
HDAC1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-855
External resources

Characterizations

HDAC1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 40967. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.103
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDAC1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 40967. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.103
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDAC1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 40967. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 55.103
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDAC1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 40967. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
HDAC1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of HDAC1 from K562 cells using 40967. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 40967. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 55 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 HDAC1 (2).jpg
HDAC1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using 40967, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
For this factor, we found two other TF (MTA2 and GATAD2A) has more peptides than targeted protein. Explanation: http://www.genecards.org/cgi-bin/carddisp.pl?gene=GATAD2A&keywords=gatad2a shows that GATAD2A has interaction with MTA2, and http://www.genecards.org/cgi-bin/carddisp.pl?gene=HDAC1&keywords=hdac1 indicated that MTA2 and HDAC1 can form core complex.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford