ENCAB487VWD
Alternate accession: ENCAB479FGN
Antibody against Homo sapiens KDM5B
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-813A
- Lot ID
- 1
- Characterized targets
- KDM5B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Lysine (K)-specific demethylase 5B; jumonji, AT rich interactive domain 1B, between 1494 and 1544.
- Aliases
- bradley-bernstein:PchAb 1241, michael-snyder:AS-1485
- External resources
Characterizations
KDM5B (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
not compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 3-8% Tris Acetate gel in 1X TA running buffer. After separation, the samples were transferred to a PVDF membrane usinga wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. 2 bands of expected sizes were detected (~170-180kDa), however other lower molecular size bands were also observed.
- Reviewer comment
- All lanes have nonspecific bands possibly from bad quality antibody. Lane one had been marked as not compliant after review on March 15 with antibody review panal.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991