ENCAB487VWD

Alternate accession: ENCAB479FGN

Antibody against Homo sapiens KDM5B

Homo sapiens
GM12878, HepG2, K562, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-813A
Lot ID
1
Characterized targets
KDM5B (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Lysine (K)-specific demethylase 5B; jumonji, AT rich interactive domain 1B, between 1494 and 1544.
Aliases
bradley-bernstein:PchAb 1241, michael-snyder:AS-1485
External resources

Characterizations

KDM5B (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 3-8% Tris Acetate gel in 1X TA running buffer. After separation, the samples were transferred to a PVDF membrane usinga wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. 2 bands of expected sizes were detected (~170-180kDa), however other lower molecular size bands were also observed.
Reviewer comment
All lanes have nonspecific bands possibly from bad quality antibody. Lane one had been marked as not compliant after review on March 15 with antibody review panal.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad