1. Antibodies
  2. Homo sapiens
  3. RCOR1

ENCAB458WED

Antibody against Homo sapiens RCOR1

Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein, H1
partially characterized
Homo sapiens
A549
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-130A
Lot ID
1
Characterized targets
RCOR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Component of an HDAC complex and a corepressor of the REST TF
Aliases
bradley-bernstein:PchAb 156
External resources

Characterizations

RCOR1 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.the strongest band was detected around the expected size (~70kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
RCOR1 (Homo sapiens)
A549H1
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (50-70kDa).
Reviewer comment
should expect 53 kDa, only H1 is 50% of the signal strength
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad