1. Antibodies
  2. Homo sapiens
  3. SAMHD1

ENCAB434HZO

Antibody against Homo sapiens SAMHD1

Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-690A
Lot ID
1
Characterized targets
SAMHD1 (Homo sapiens)
Host
rabbit

Characterizations

SAMHD1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against SAMHD1 in HepG2 whole cell lysate using SAMHD1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAMHD1. SAMHD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
SAMHD1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against SAMHD1 in K562 whole cell lysate using SAMHD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SAMHD1. SAMHD1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Reviewer comment
Checked with Eric Mendenhall and he agreed with the lab’s explanation: Based on the explanation we got from the lab, these are all CRISPRi based knockouts (unlike the previous RNAi based knockouts) where the lab introduces a stop codon, thus preventing protein synthesis altogether. And as a result, they are able to detect only the protein knockdown rates, but the RNA levels still might not be changing and thus they report XX% k/d using qRT-PCR since they couldn’t even detect anything. This is unlike the RNAi based silencing methods.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn