ENCAB412ETD
Antibody against Homo sapiens DDX43
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A302-328A
- Lot ID
- 1
- Characterized targets
- DDX43 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- between 275 and 325
- External resources
Characterizations
DDX43 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of K562 whole cell lysate using the DDX43 specific antibody, A302-328A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment using a normal IgG antibody, respectively. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX43-specific antibody, A302-328A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U41HG009889
- Download
- Bethyl_A302-328A_1_DDX43.png
DDX43 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against DDX43 in K562 whole cell lysate using DDX43 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX43. DDX43 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- DDX43-K562-CRISPR-A302-328A.png