ENCAB412ETD

Antibody against Homo sapiens DDX43

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-328A
Lot ID
1
Characterized targets
DDX43 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
between 275 and 325
External resources

Characterizations

DDX43 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the DDX43 specific antibody, A302-328A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment using a normal IgG antibody, respectively. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX43-specific antibody, A302-328A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
DDX43 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against DDX43 in K562 whole cell lysate using DDX43 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX43. DDX43 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn