1. Antibodies
  2. Homo sapiens
  3. POLR2G

ENCAB392AZR

Alternate accession: ENCAB386AXO

Antibody against Homo sapiens POLR2G

Homo sapiens
HepG2
characterized to standards
Homo sapiens
K562
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
GeneTex
Product ID
GTX108874
Lot ID
40023
Characterized targets
POLR2G (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Aliases
xiang-dong-fu:POLR2G
External resources

Characterizations

POLR2G (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using POLR2G specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-POLR2GpAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
POLR2G (Homo sapiens)
HepG2K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using POLR2G specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-POLR2G antibody (lanes under 'POLR2G').
Submitter comment
This antibody was given and exemption for K562. The data was clean and the pattern is reliable.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
POLR2G (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against POLR2G in K562 whole cell lysate using POLR2G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2G.POLR2G protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn