1. Antibodies
  2. Homo sapiens
  3. JARID2

ENCAB385UAF

Antibody against Homo sapiens JARID2

Homo sapiens
K562, GM12878, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-134548
Lot ID
1
Characterized targets
JARID2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Nuclear transcriptional repressor. Jumonji Antibody (H-260) is a rabbit polyclonal IgG provided at 200 µg/ml. Epitope corresponding to amino acids 961-1220 mapping near the C-terminus of Jumonji of human origin.
Antigen sequence
EDVVHTLLQANGTPGLQMLESNVMISPEVLCKEGIKVHRTVQQSGQFVVCFPGSFVSKVCCGYSVSETVHFATTQWTSMGFETAKEMKRRHIAKPFSMEKLLYQIAQAEAKKENGPTLSTISALLDELRDTELRQRRQLFEAGLHSSARYGSHDGSSTVADGKKKPRKWLQLETSERRCQICQHLCYLSMVVQENENVVFCLECALRHVEKQKSCRGLKLMYRYDEEQIISLVNQICGKVSGKNGSIENCLSKPTPKRGP
Aliases
bradley-bernstein:PchAb 438
External resources

Characterizations

JARID2 (Homo sapiens)
K562GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Nuclear lysates from GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~80kDa).
Reviewer comment
KCO: Captions state that expected size should be ~80kDA, yet immunoblot image labels otherwise (shows that it's suppose to be ~150-160kDa). Either way, the strongest band under K562 lane is ~64kDa, which is off in either direction of expected band size.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad