ENCAB337YOC

Antibody against Homo sapiens WDR77

Homo sapiens
HepG2, K562, GM12878, H1, endothelial cell of umbilical vein, A549
partially characterized
Status
released
Source (vendor)
Cell Signaling
Product ID
2018BF
Lot ID
2
Characterized targets
WDR77 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
Non-catalytic component of a methyltransferase complex. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser225 of human MEP50.
Aliases
bradley-bernstein:PchAb 1255
External resources

Characterizations

WDR77 (Homo sapiens)
A549H1
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
WDR77 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad