ENCAB337YOC
Antibody against Homo sapiens WDR77
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein, A549, H1
partially characterized
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 2018BF
- Lot ID
- 2
- Characterized targets
- WDR77 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Non-catalytic component of a methyltransferase complex. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser225 of human MEP50.
- Aliases
- bradley-bernstein:PchAb 1255
- External resources
Characterizations
WDR77 (Homo sapiens)
A549H1
compliant
- Caption
- Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- WDR77_MEP50_CST_2018BF_Lot2_V2.png
WDR77 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
compliant
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- WDR77_MEP50_CST_2018BF_Lot2_WB.png