ENCAB337YOC

Antibody against Homo sapiens WDR77

Homo sapiens
endothelial cell of umbilical vein, K562, H1-hESC, HepG2, A549, GM12878
partially characterized
Status
released
Source (vendor)
Cell Signaling
Product ID
2018BF
Lot ID
2
Characterized targets
WDR77 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
Non-catalytic component of a methyltransferase complex. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser225 of human MEP50.
Aliases
bradley-bernstein:PchAb 1255
External resources

Characterizations

WDR77 (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
WDR77 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad