1. Antibodies
  2. Homo sapiens
  3. APEX1

ENCAB328DHC

Antibody against Homo sapiens APEX1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
MA1-440
Lot ID
TB260470
Characterized targets
APEX1 (Homo sapiens)
Host
mouse

Characterizations

APEX1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against APEX1 in HepG2 whole cell lysate using APEX1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against APEX1. APEX1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
APEX1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against APEX1 in K562 whole cell lysate using APEX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against APEX1. APEX1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
APEX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the APEX1 specific antibody, MA1-440. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the APEX1-specific antibody, MA1-440. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
Lab comment See ENCAB654PTX for an approved example.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD