1. Antibodies
  2. Homo sapiens
  3. MBNL1

ENCAB328AVR

Antibody against Homo sapiens MBNL1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA5-19739
Lot ID
TK2672742
Characterized targets
MBNL1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Muscleblind-like 1.

Characterizations

MBNL1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against MBNL1 in K562 whole cell lysate using MBNL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against MBNL1. MBNL1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
MBNL1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the MBNL1 specific antibody, PA5-19739. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the MBNL1-specific antibody, PA5-19739. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD