1. Antibodies
  2. Homo sapiens
  3. RPS6

ENCAB305KJD

Antibody against Homo sapiens RPS6

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-556A
Lot ID
2
Characterized targets
RPS6 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Between 100 and 150
External resources

Characterizations

RPS6 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the RPS6 specific antibody, A300-556A. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the RPS6-specific antibody, A300-556A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
The detected band is 4kDa higher than expected protein size at most. That's within 20%. Multiple bands are seen. But the band at expected size is more than 50% or the signal thus compliant according to standards.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
RPS6 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
RPS6 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against RPS6 in K562 whole cell lysate using RPS6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPS6. RPS6 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn