ENCAB303CHK
Antibody against Homo sapiens SAFB2
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-112A
- Lot ID
- 1
- Characterized targets
- SAFB2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- xiang-dong-fu:SAFB2
- External resources
Characterizations
SAFB2 (Homo sapiens)
K562
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using SAFB2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-SAFB2 antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Reviewer comment
- Review Panel Agreed that although not ideal, this was reasonable.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A301-112A_1_SAFB2.png
SAFB2 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using SAFB2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SAFB2 antibody (lanes under 'SAFB2').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A301-112_1_SAFB2.png
SAFB2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against SAFB2 in HepG2 whole cell lysate using SAFB2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SAFB2. SAFB2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SAFB2-HEPG2.png
SAFB2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against SAFB2 in K562 whole cell lysate using SAFB2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SAFB2. SAFB2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitter comment
- WB% numbers are switched for lane 3/4?
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SAFB2-K562.png