ENCAB284TTY

Antibody against Homo sapiens EP300

Homo sapiens
GM12878, K562
characterized to standards
Homo sapiens
prostate gland, breast epithelium, gastroesophageal sphincter, upper lobe of left lung, omental fat pad, esophagus muscularis mucosa, sigmoid colon, transverse colon, vagina, testis, ovary, stomach, suprapubic skin, tibial nerve, subcutaneous adipose tissue
characterized to standards with exemption
Homo sapiens
HepG2, MCF-7
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-585
Lot ID
H3115
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
epitope mapping at the C-terminus of p300 of human origin
External resources

Characterizations

EP300 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Reviewer comment
Compliant Mass Spec for a secondary characterization of ENCAB284TTY
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
EP300 (Homo sapiens)
K562GM12878HepG2MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Whole cell lysates of K562, GM12878, MCF7 and HepG2 were immunoprecipitated using the primary antibody (Santa Cruz Biotechnology; sc-585; Lot# H3115). The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. The membrane was probed with the primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody. The resulting bands were visualized with SuperSignal West Femto Solution (Thermo Scientific). Protein Marker (PM) is labeled in kDa. The approximate size of EP300 is ~300 kDa.
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
EP300 (Homo sapiens)
transverse colonstomachsubcutaneous adipose tissueomental fat padsigmoid colonesophagus muscularis mucosagastroesophageal sphincterupper lobe of left lungsuprapubic skinovarytestisprostate glandbreast epitheliumvaginatibial nerve
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
The ENCODE Binding Working Group finds for some valuable tissues that recreating a primary on well characterized antibodies is not cost effective. Therefore, they allow exemption from standards for these tissues. Compliant characterizations in other cell lines may be used instead.
Submitter comment
The lab is asking for an exemption for transverse colon, stomach, subcutaneous adipose, and omental fat pad tissues due to the lack of resources to make a primary characterization for them
Reviewer comment
--
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
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