ENCAB278ULX

Antibody against Homo sapiens SRSF9

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN081PW
Lot ID
001
Characterized targets
SRSF9 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Peptide, N-terminus of human SRSF9
External resources

Characterizations

SRSF9 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using SRSF9 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 20% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 20% IP enrichment using rabbit polyclonal anti-SRSF9 antibody (lanes under 'SRSF9').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
SRSF9 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the SRSF9-specific antibody RN081PW. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using SRSF9 antibody. Lane 4: Immunoprecipitated material using SRSF9 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 25.54 kDa.
Reviewer comment
This is a duplicate to the other characterization
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
SRSF9 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using SRSF9 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 20% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 20% IP enrichment using rabbit polyclonal anti-SRSF9 antibody (lanes under 'SRSF9').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
SRSF9 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of SRSF9 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SRSF9(SRp30c)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
SRSF9 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of MCF7 whole cell lysate using SRSF9 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SRSF9 pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
SRSF9 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody RN081PW, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
SRSF9 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against SRSF9 in K562 whole cell lysate using SRSF9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF9.SRSF9 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
SRSF9 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against SRSF9 in HepG2 whole cell lysate using SRSF9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF9.SRSF9 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn