ENCAB251NRY

Antibody against Homo sapiens CARM1

Homo sapiens
H1-hESC, A549
partially characterized
Status
released
Source (vendor)
Abcam
Product ID
ab50214
Lot ID
GR31994-2
Characterized targets
CARM1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
coactivator-associated arginine methyltransferase 1 (CARM1); Protein arginine N-methyltransferase 4 (PRMT4)
Aliases
bradley-bernstein:PchAb 249
External resources

Characterizations

CARM1 (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected within the expected size range (41kDa, 63kDa and 65kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad