ENCAB251NRY
Antibody against Homo sapiens CARM1
Homo sapiens
A549, H1
partially characterized
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab50214
- Lot ID
- GR31994-2
- Characterized targets
- CARM1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- coactivator-associated arginine methyltransferase 1 (CARM1); Protein arginine N-methyltransferase 4 (PRMT4)
- Aliases
- bradley-bernstein:PchAb 249
- External resources
Characterizations
CARM1 (Homo sapiens)
A549H1
Method: immunoblot
compliant
- Caption
- Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected within the expected size range (41kDa, 63kDa and 65kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991