1. Antibodies
  2. Homo sapiens
  3. GLRX3

ENCAB219JBV

Antibody against Homo sapiens GLRX3

Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
GeneTex
Product ID
GTX118339
Lot ID
40310
Characterized targets
GLRX3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

GLRX3 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB characterization of GLRX3 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal TXNL2 antibody[N2C3]. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
GLRX3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against GLRX3 in HepG2 whole cell lysate using GLRX3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GLRX3. GLRX3 protein appears as the green band, Beta-actin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GLRX3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using GLRX3/TXNL2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal TXNL2 [N2C3] antibody (lanes under 'TXNL2').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
GLRX3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against TXNL2 in K562 whole cell lysate using TXNL2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TXNL2. TXNL2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn