ENCAB214FIO
Antibody against Homo sapiens SSB
Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN074PW
- Lot ID
- 001
- Characterized targets
- SSB (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide, C-terminus of human SSB
- External resources
Characterizations
SSB (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using SSB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SSB antibody (lanes under 'SSB').
- Submitter comment
- Steven Blue, Yeo Lab: The smudginess in lane 4 of the HepG2 image is most likely due to overexposure and/or an irregularity in the gel.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN074PW_001_SSB.png
SSB (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using SSB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SSB antibody (lanes under 'SSB').
- Submitter comment
- Steven Blue, Yeo Lab: The smudginess in lane 4 of the HepG2 image is most likely due to overexposure and/or an irregularity in the gel.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN074PW_001_SSB.png
SSB (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using SSB specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SSB(La)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN074PW_001_SSB.png
SSB (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against SSB in HepG2 whole cell lysate using SSB specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SSB. SSB protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SSB-HEPG2.png