ENCAB211WTE

Antibody against Homo sapiens H3K36me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Diagenode
Product ID
pAb-192-050
Lot ID
A1847-001P
Characterized targets
H3K36me3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 724
External resources

Characterizations

H3K36me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K36me3 antibody was the correct one.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K36me3 (Homo sapiens)
HeLa-S3
Method: immunoblot
compliant
Caption
We performed a WB using 20ug of nuclear lysate from HeLa nuclear extract. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in Superblock for 1 hour, Antibodies diluted in Superblock imaged in ChemiDoc.
Reviewer comment
The characterization was completed for HeLa-S3 only as K562 was not subject to be tested by the H3K36me3 antibody in question, even though it is represented in panel A (but not panel B). However, this is a sufficient characterization for all human samples.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad