ENCAB182PZR
Antibody against Homo sapiens ZNF217
Homo sapiens
MCF-7, K562, GM12878
characterized to standards with exemption
Homo sapiens
HepG2
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-265A
- Lot ID
- 1
- Characterized targets
- ZNF217 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1029
- External resources
Characterizations
ZNF217 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-265A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 115.272.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1035K562ZNF217(A303-265)-1.jpg
ZNF217 (Homo sapiens)
MCF-7
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-265A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 115.272
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1075_08_ZNF217_A303-265A.jpg
ZNF217 (Homo sapiens)
MCF-7
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-265A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ZNF217 (Homo sapiens)
HepG2
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-265A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 115.272.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 5.jpg
ZNF217 (Homo sapiens)
GM12878
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-265A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 115.272.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Scan_20170420 (4).jpg
ZNF217 (Homo sapiens)
exempt from standards
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-265A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- CHD4 and HDAC1 (55KD), HDAC2 (55KD) are in one complex, ZNF217 and HDAC1, HDAC2 are components of BHC complex, CHD4 are also part of this complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=HDAC2&keywords=hdac2 and http://www.genecards.org/cgi-bin/carddisp.pl?gene=HDAC1&keywords=hdac1. HDAC1 and HDAC2 are not in the same gel slice with CHD4 and ZNF217. Both HNRNPUL1 and ZNF217 have interactions with ELAVL1 (36KD), http://thebiogrid.org/113547/summary/homo-sapiens/znf217.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html. ELAVL1 is not in the same gel slice with HNRNPUL1 and ZNF217. Both ZNF217 and KDM1A are components of histone deacetylase complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=ZNF217&keywords=znf217.
- Reviewer comment
- Although chromatin-associated protein CHD4 is ranked higher by enrichment than ZNF217, the submitters note that CHD4 and ZNF217 may be part of the same complex.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZNF217_A303-265A_final.pdf