ENCAB181ALQ

Antibody against Homo sapiens CTCF

  • Status: released
characterized to standards
Homo sapiens
HeLa-S3
characterized to standards with exemption
Homo sapiens
thyroid gland
Source (vendor)
Cell Signaling
Product ID
3418BF
Lot ID
2
Host
rabbit
Clonality
monoclonal
Antigen description
carboxy terminus of the human CTCF protein
Aliases
bradley-bernstein:PchAb 1144

Characterizations

CTCF (Homo sapiens)
Method: ChIP-seq comparison
  • compliant
Caption
This validation relies on the use of two different antibodies raised against different, non-overlapping peptides, both derived from the sequence of human CTCF, and the discovery of highly correlated patterns of enrichment by ChIP-Seq in K562 cells. The polyclonal antibody being validated (ENCAB000AXX) was raised against a 17 residue long synthetic peptide located 52 residues away from the C-terminus of CTCF. In contrast, the monoclonal antibody was raised against a peptide derived from the extreme C-terminus of CTCF. Overall correlation score = 0.976
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CTCF (Homo sapiens)
HeLa-S3
Method: immunoblot
  • compliant
Caption
We performed a WB using 25ug of nuclear extract from HeLa cells. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in TBST/ 5% BSA for 1 hour, Antibodies diluted in TBST/ 5% BSA, exposure in ChemiDoc.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CTCF (Homo sapiens)
thyroid gland
Method: immunoprecipitation
Attachment from submitter
  • exempt from standards
Submitter comment
This was cleared by the Antibody Committee.
Reviewer comment
This was cleared by the Antibody Committee.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
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