ENCAB181ALQ

Homo sapiens

HeLa-S3

characterized to standards

Homo sapiens

thyroid gland

characterized to standards with exemption

Method: ChIP-seq comparison

compliant

Caption: This validation relies on the use of two different antibodies raised against different, non-overlapping peptides, both derived from the sequence of human CTCF, and the discovery of highly correlated patterns of enrichment by ChIP-Seq in K562 cells. The polyclonal antibody being validated (ENCAB000AXX) was raised against a 17 residue long synthetic peptide located 52 residues away from the C-terminus of CTCF. In contrast, the monoclonal antibody was raised against a peptide derived from the extreme C-terminus of CTCF. Overall correlation score = 0.976
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: CTCF PchAb 1144 SAV.pdf
Documents: encode:TF_Antibody_Characterization_ENCODE3_May2016.pdf

HeLa-S3

Method: immunoblot

compliant

Caption: We performed a WB using 25ug of nuclear extract from HeLa cells. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in TBST/ 5% BSA for 1 hour, Antibodies diluted in TBST/ 5% BSA, exposure in ChemiDoc.
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: CTCF_ Cell Signaling_Part 3417_Lot 2_WB_PchAb 1145 clone D1A7.pdf
Documents: encode:TF_Antibody_Characterization_ENCODE3_May2016.pdf

thyroid gland

Method: immunoprecipitation

exempt from standards