ENCAB181ALQ

Antibody against Homo sapiens CTCF

Homo sapiens
HeLa-S3
characterized to standards
Homo sapiens
thyroid gland
characterized to standards with exemption
Status
released
Source (vendor)
Cell Signaling
Product ID
3418BF
Lot ID
2
Host
rabbit
Clonality
monoclonal
Antigen description
carboxy terminus of the human CTCF protein
Aliases
bradley-bernstein:PchAb 1144

Characterizations

CTCF (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of two different antibodies raised against different, non-overlapping peptides, both derived from the sequence of human CTCF, and the discovery of highly correlated patterns of enrichment by ChIP-Seq in K562 cells. The polyclonal antibody being validated (ENCAB000AXX) was raised against a 17 residue long synthetic peptide located 52 residues away from the C-terminus of CTCF. In contrast, the monoclonal antibody was raised against a peptide derived from the extreme C-terminus of CTCF. Overall correlation score = 0.976
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CTCF (Homo sapiens)
HeLa-S3
Method: immunoblot
compliant
Caption
We performed a WB using 25ug of nuclear extract from HeLa cells. Extract was resolved by electrophoresis on a 4-12% Bis-Tris, 1X MES running buffer iBlot transfer, nitrocellulose, P3 for 8 minutes, Blocked in TBST/ 5% BSA for 1 hour, Antibodies diluted in TBST/ 5% BSA, exposure in ChemiDoc.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CTCF (Homo sapiens)
thyroid gland
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Submitter comment
This was cleared by the Antibody Committee.
Reviewer comment
This was cleared by the Antibody Committee.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
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