ENCAB140BWE

Antibody against Homo sapiens H3K4me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
9751BC
Lot ID
9
Characterized targets
H3K4me3 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Aliases
bradley-bernstein:PchAb 1360

Characterizations

H3K4me3 (Homo sapiens)
Method: dot blot assay
compliant
Caption
We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K4me3 antibody was the correct one.\n
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K4me3 (Homo sapiens)
H1-hESCHepG2K562
Method: immunoblot
compliant
Caption
Nuclear lysates from H1-hESC (10ug, lane 2), HepG2 (10ug, lane 3), and K562 (10ug, lane 4) cells, were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iBlot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~17kDa).
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad