ENCAB140BWE

Homo sapiens

any cell type or tissue

characterized to standards

Method: dot blot assay

compliant

Caption: We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K4me3 antibody was the correct one.\n
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: UM1HG009390
Download: H3K4me3_Cell Signaling_Part 9751BF_Lot 9 _ DB_PchAb 1360.pdf
Documents: encode:Histone_Modification_Antibody_Characterization_ENCODE3_Oct2016.pdf

H1HepG2K562

Method: immunoblot

compliant

Caption: Nuclear lysates from H1-hESC (10ug, lane 2), HepG2 (10ug, lane 3), and K562 (10ug, lane 4) cells, were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iBlot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~17kDa).
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: UM1HG009390
Download: H3K4me3_Cell Signaling_Part 9751BF_Lot 9 _WB_PchAb 1360.pdf
Documents: encode:Histone_Modification_Antibody_Characterization_ENCODE3_Oct2016.pdf