ENCAB118PND

Antibody against Homo sapiens ZFP36

Homo sapiens
GM12878, A549, K562, HeLa-S3, HepG2
partially characterized
Status
released
Source (vendor)
Sigma
Product ID
T5327
Lot ID
078K4810
Characterized targets
ZFP36 (Homo sapiens)
Host
rabbit
Aliases
michael-snyder:ZFP36-human_T5327_078K4810
External resources

Characterizations

ZFP36 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized antibody that has been in use without issue by the community for many years.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments.
Reviewer comment
This is a well characterized antibody already used in many ENCODE experiments.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
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ZFP36 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody T5327. Molecular Weight: 50.0 kDa
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZFP36 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody T5327. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 50.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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