ENCAB118PND
Antibody against Homo sapiens ZFP36
Homo sapiens
A549, GM12878, K562, HeLa-S3, HepG2
partially characterized
- Status
- released
- Source (vendor)
- Sigma
- Product ID
- T5327
- Lot ID
- 078K4810
- Characterized targets
- ZFP36 (Homo sapiens)
- Host
- rabbit
- Aliases
- michael-snyder:ZFP36-human_T5327_078K4810
- External resources
Characterizations
ZFP36 (Homo sapiens)
A549
Method: immunoprecipitation
exempt from standards
- Caption
- Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized antibody that has been in use without issue by the community for many years.
- Submitter comment
- This is a well characterized antibody already used in many ENCODE experiments.
- Reviewer comment
- This is a well characterized antibody already used in many ENCODE experiments.
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- exempted.png
ZFP36 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody T5327. Molecular Weight: 50.0 kDa
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZFP36(TTP)-WB-260.jpg
ZFP36 (Homo sapiens)
K562
Method: immunoprecipitation
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody T5327. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 50.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZFP36.jpg