1. Antibodies
  2. Homo sapiens
  3. JUN

ENCAB118DQK

Antibody against Homo sapiens JUN

Homo sapiens
MCF-7, K562, A549
characterized to standards with exemption
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-1694
Lot ID
E2512
Characterized targets
JUN (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
epitope corresponding to amino acids 1-79 mapping at the N-terminus of c-Jun of human origin
Aliases
michael-snyder:AS-28
External resources

Characterizations

JUN (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-1694. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Molecular Weight: 39 kDa.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
JUN (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody sc-1694 (Lot: E2512). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 35.7.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
JUN (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
A new secondary characterization is not required as one exists for ENCAB000AER which is a lot of the same antibody (sc-1694). The primary characterization of K562 (https://www.encodeproject.org/antibody-characterizations/b8a9f7da-59b3-4994-8978-8c19294c4f11/) for this lot shows the same banding pattern as the primary characterization (https://www.encodeproject.org/antibody-characterizations/42adb54e-fade-431a-ad8c-61dc88d7e11a/) for ENCAB000AER.
Submitter comment
This is a new lot of a previously characterized antibody ENCAB000AER and we think no further characterization is necessary beyond the primary if the banding pattern is consistent with the old.
Reviewer comment
The banding in K562 is consistent between new and old lots so a new secondary characterization is not necessary according to the standards.
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
JUN (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-1694. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford