ENCAB101KHB
Antibody against Homo sapiens H3K27ac
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39133
- Lot ID
- 1613007
- Characterized targets
- H3K27ac (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.
- Aliases
- bradley-bernstein:PchAb 1099
- External resources
Characterizations
H3K27ac (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K27ac antibody was the correct one; According to vendor lots 25812006 and 01613007 are from the same parent batch.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27ac (Homo sapiens)
K562
Method: immunoblot
compliant
- Caption
- 0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 30ug of K562 Whole Cell Lysate (Abcam, ab7911) were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and WCL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in WCL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected. According to vendor lots 25812006 and 01613007 are from the same parent batch.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991