ENCAB097MPS
Antibody against Homo sapiens NONO
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-166702
- Lot ID
- C1810
- Characterized targets
- NONO (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG2b
- Antigen description
- Specific for an epitope mapping between amino acids 370-405 near the C-terminus of p54/nrb of human origin
- External resources
Characterizations
NONO (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against NONO in HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NONO. NONO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- NONO-HEPG2-FU'S.png
NONO (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- NONO_HepG2_sc.png
NONO (Homo sapiens)
K562
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- NONO_K562_sc.png