ENCAB097MPS

Antibody against Homo sapiens NONO

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-166702
Lot ID
C1810
Characterized targets
NONO (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2b
Antigen description
Specific for an epitope mapping between amino acids 370-405 near the C-terminus of p54/nrb of human origin
External resources

Characterizations

NONO (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against NONO in HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NONO. NONO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
NONO (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
NONO (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD