ENCAB064PJY
Antibody against Homo sapiens CREBBP
Homo sapiens
K562
not characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 7389S
- Lot ID
- 1
- Characterized targets
- CREBBP (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Antigen description
- cAMP-response element binding protein binding protein
- Aliases
- bradley-bernstein:PchAb 1261
- External resources
Characterizations
CREBBP (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane. We assume that since the protein is relatively big, it goes through degradation and therefore we get more bands in unexpected areas.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- cbp_cell_signaling_7389S_1.png