1. Antibodies
  2. Homo sapiens
  3. METTL1

ENCAB061GXO

Antibody against Homo sapiens METTL1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA5-80810
Lot ID
UL2893376
Characterized targets
METTL1 (Homo sapiens)
Host
rabbit

Characterizations

METTL1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against METTL1 in HepG2 whole cell lysate using METTL1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against METTL1. METTL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
METTL1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against METTL1 in K562 whole cell lysate using METTL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against METTL1. METTL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
METTL1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the METTL1 specific antibody, PA5-80810. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the METTL1-specific antibody, PA5-80810. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD