1. Antibodies
  2. Homo sapiens
  3. GSPT2

ENCAB053JZL

Antibody against Homo sapiens GSPT2

Homo sapiens
at least one cell type or tissue, K562
not characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN119PW
Lot ID
001
Characterized targets
GSPT2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
External resources

Characterizations

GSPT2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
not compliant
Caption
Western blot following CRISPR against GSPT2 in K562 whole cell lysate using GSPT2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GSPT2. GSPT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GSPT2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
IP-WB analysis of K562 whole cell lysate using the GSPT2 specific antibody, RN119PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GSPT2-specific antibody, RN119PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Reviewer comment
Lane changed to 3 (2022/08/08).
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
GSPT2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using GSPT2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-GSPT2 (eRF3b) (Human) pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD