1. Antibodies
  2. Homo sapiens
  3. SF3B1

ENCAB022ALQ

Antibody against Homo sapiens SF3B1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab66774
Lot ID
GR216813-1
Characterized targets
SF3B1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
Protein A
Isotype
IgG
Antigen description
Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human SAP155
Antigen sequence
MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR
External resources

Characterizations

SF3B1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B1 antibody (lanes under 'SF3B1').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
SF3B1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against SF3B1 in HepG2 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
Download
 SF3B1-HEPG2.png
SF3B1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against SF3B1 in K562 whole cell lysate using SF3B1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SF3B1.SF3B1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn