1. Antibodies
  2. Homo sapiens
  3. GARS

ENCAB009SWM

Antibody against Homo sapiens GARS

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
MA5-26714
Lot ID
UA2693064
Characterized targets
GARS (Homo sapiens)
Host
mouse

Characterizations

GARS (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against GARS in HepG2 whole cell lysate using GARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GARS. GARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GARS (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against GARS in K562 whole cell lysate using GARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GARS. GARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
GARS (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the GARS specific antibody, MA5-26714. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the GARS-specific antibody, MA5-26714. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD