1. Antibodies
  2. Homo sapiens
  3. IRF2

ENCAB001RIV

Antibody against Homo sapiens IRF2

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-13042
Lot ID
I1603
Characterized targets
IRF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
michael-snyder:IRF2-human_sc-13042_I1603
External resources

Characterizations

IRF2 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target IRF2 is represented by the attached position weight matrix (PWM) derived from ENCFF284BAX. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.658136505228 Global enrichment Z-score: 7.31182165197 Positional bias Z-score: 11.7100005936 Peak rank bias Z-score: 10.4205916377 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
IRF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-13042. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 39.354
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IRF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using sc-13042, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Reviewer comment
There are only 4 peptides from distinct proteins detected and all are equally ranked...
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
IRF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of IRF2 from K562 cells using sc-13042. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with sc-13042. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~50kD.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 IRF2_IP.png