ENCAB000BRO

Antibody against Homo sapiens SIRT1

Homo sapiens
HepG2, K562
partially characterized
Status
released
Source (vendor)
Active Motif
Product ID
39354
Lot ID
22808001
Characterized targets
SIRT1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein G
Isotype
IgG1
Antigen description
Raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1
External resources

Characterizations

SIRT1 (Homo sapiens)
K562HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
An immunoprecipitation followed by Western blot was performed with Active Motif 39354 (lot 22808001) antibody against SIRT1 in K562 and HepG2 whole cell lysate along with a control IP with normal IgG from the same host species as the test antibody (mouse, Santa Cruz sc-2025). The expected size of SIRT1 is 120 kDa. A band of ~100 kDa was detected in the test IP and not in the control IP. The experiment was repeated and this band was analyzed by mass spec in the secondary validation.
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB