1. Antibodies
  2. Homo sapiens
  3. FOXP1

ENCAB000BMD

Antibody against Homo sapiens FOXP1

Homo sapiens
K562, HepG2
characterized to standards with exemption
Homo sapiens
GM12878, HeLa-S3, MCF-7, liver
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
HPA003876
Lot ID
A27581
Characterized targets
FOXP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Forkhead box protein P1 recombinant protein epitope signature tag (PrEST)
Antigen sequence
QMQQLQQQHLLSLQRQGLLTIQPGQPALPLQPLAQGMIPTELQQLWKEVTSAHTAEETTGNNHSSLDLTTTCVSSSAPSKTSLIMNPHASTNGQLSVHTPKRESLSHEEHPHSHPLYGHGVCKWPGCEAVCEDFQSFLKHLNS
External resources

Characterizations

FOXP1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody HPA003876, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
FUS, DDX5, G3BP1, ILF3, DDX3X and TAF15 are not DNA binding TFs. FOXP4 has interaction with FOXP1, http://thebiogrid.org/117989/summary/homo-sapiens/foxp1.html.
Reviewer comment
The ENCODE Antibody Review panel decided to exempt this characterization on 12/19/16, agreeing with the submitters that the proteins ranked about FOXP1 are not sequence-specific TFs.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXP1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3, MCF7 and Liver using the antibody HPA003876. Molecular mass: 75317 Da
Reviewer comment
Band is either missing or is not 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
FOXP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody HPA003876 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expecyed size ~75 kDa.
Submitter comment
FOXP1 has 8 isoforms, including 75KD, 65KD and 54KD, it may explain the multiple bands.
Reviewer comment
Multiple bands and marked band is not 50% of total signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
FOXP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody HPA003876. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 75.3.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford