ENCAB000BLL

Antibody against Homo sapiens NRF1

Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
HepG2, K562
not characterized to standards
Status
released
Source (vendor)
CDI
Product ID
R157.1.3H1
Lot ID
011513.RAP
Characterized targets
NRF1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG2b
External resources

Characterizations

NRF1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target NRF1 is represented by the attached position weight matrix (PWM) derived from ENCFF803TIA. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.91960821639, Global enrichment Z-score: 6.36881734605, Positional bias Z-score: 14.4329962635, Peak rank bias Z-score: 14.8942315546, Enrichment rank: 1.0.
Submitted by
Aditi Narayanan
Lab
Richard Myers, HAIB
Download
NRF1 (Homo sapiens)
K562HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
An immunoprecipitation followed by Western blot was performed with CDI R157.1.3H1 (lot 011513-RAP) antibody against NRF1 in HepG2 and K562 whole cell lysate. The expected size of NRF1 is 84 kDa. A band of ~75 kDa was detected. The experiment was repeated and this band was analyzed by mass spec in the secondary validation.
Submitter comment
non specific bands and no IgG control
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB
NRF1 (Homo sapiens)
Method: immunoblot
Attachment from submitter
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, MCF7, Liver using the antibody R157.1.3H1.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
NRF1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody R157.1.3H1 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford