ENCAB000BGC
Antibody against Homo sapiens TIA1
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN014P
- Lot ID
- 001
- Characterized targets
- TIA1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- KLH-conjugated synthetic peptide NGSMLPNQPSGYRVAGYETQ ï¼367-386 aa)
- Antigen sequence
- NGSMLPNQPSGYRVAGYETQ
- External resources
Characterizations
TIA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using TIA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TIA1 antibody (lanes under 'TIA1').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN014P_001_TIA1.png
TIA1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western blot analysis of K562 whole cell lysate using TIA1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-TIA1pAb. The last three lanes are the same pattern, but with IgG control instead of TIA1 antibody.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN014P_001_TIA1.png
TIA1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against Tia1 in K562 and HepG2 whole cell lysate using Tia1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against Tia1. Lanes 5-7 follow the same pattern, but in HepG2. Tia1 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Brenton Graveley
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- TIA1_Secondary_Western.jpg