ENCAB000BGC

Antibody against Homo sapiens TIA1

Homo sapiens
HepG2, K562
characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN014P
Lot ID
001
Characterized targets
TIA1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
KLH-conjugated synthetic peptide NGSMLPNQPSGYRVAGYETQ (367-386 aa)
Antigen sequence
NGSMLPNQPSGYRVAGYETQ
External resources

Characterizations

TIA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using TIA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TIA1 antibody (lanes under 'TIA1').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
TIA1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western blot analysis of K562 whole cell lysate using TIA1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-TIA1pAb. The last three lanes are the same pattern, but with IgG control instead of TIA1 antibody.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
TIA1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against Tia1 in K562 and HepG2 whole cell lysate using Tia1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against Tia1. Lanes 5-7 follow the same pattern, but in HepG2. Tia1 protein appears as the green band, GAPDH serves as a control and appears in red.
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn