ENCAB000BFB
Antibody against Homo sapiens RB1
Homo sapiens
GM12878, K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue, HEK293T
partially characterized
Homo sapiens
HeLa-S3, IMR-90, heart
not characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9313S
- Lot ID
- 2
- Characterized targets
- RB1 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to central residues of mouse Rb.
- External resources
Characterizations
RB1 (Homo sapiens)
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1060_9_Rb_9313S.jpg
RB1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1070_3_Rb_9313S.jpg
RB1 (Homo sapiens)
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 110.0
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1032-6.jpg
RB1 (Homo sapiens)
HEK293T
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T using the antibody 9313S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 110.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1038_24-RB1-9313S.JPG
RB1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 9313S. Molecular mass: 106159 Da
- Reviewer comment
- Band is either missing (lanes 3 and 6) or is not 50% of signal (lanes 2 and 5). Lanes 1 and 4 are compliant.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RB1_9313S_WB_a.jpg
RB1 (Homo sapiens)
HepG2
compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody 9313S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~106 kDa.
- Reviewer comment
- Multiple bands but major band within 20% of expected size and >50% of total signal in the lane.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RB1_9313S_WB_b.jpg
RB1 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 9313S, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
- Reviewer comment
- Need to provide full peptide list.
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- Rb_final Sheet1.pdf
RB1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation of Rb from K562 cells using 9313S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 9313S. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~110 kD.
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- Rb_IP.png