ENCAB000BED

Antibody against Homo sapiens TCF7L2

Homo sapiens
HeLa-S3, K562, MCF-7, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, IMR-90, heart
not characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
2569S
Lot ID
2
Characterized targets
TCF7L2 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to residues surrounding Leu330 of human TCF4.
External resources

Characterizations

TCF7L2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 2569S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.919
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 2569S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.919
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target TCF7L2 is represented by the attached position weight matrix (PWM) derived from ENCFF812DEY. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.956583594 Global enrichment Z-score: 4.002704119 Positional bias Z-score: 5.622301148 Peak rank bias Z-score: 1.309210359 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 2569S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.919
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
TCF7L2 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 2569S. Molecular mass: 67919 Da
Reviewer comment
Band is missing (Lanes 1, 2, 5, 6). Lanes 3 and 4 are compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 2569S .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size is 67-68 kD.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford