ENCAB000BDU
Antibody against Homo sapiens STAT6
Homo sapiens
GM12878, K562, HepG2, HeLa-S3, MCF-7, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-374021
- Lot ID
- G2712
- Characterized targets
- STAT6 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG2b
- Antigen description
- Epitope mapping between amino acids 799-823 at the C-terminus of Stat6 of mouse origin
- External resources
Characterizations
STAT6 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
not compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-374021.
- Reviewer comment
- Multiple bands with none making up >50% of the total signal. Expected size (~94kD) is missing from image and caption. May possibly be rescued with a siRNA knockdown
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- STAT6_sc-374021_WB_a.jpg
STAT6 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody S5061 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- No band is 50% of signal, though which band/expected size is unknown
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- STAT6_sc-374021_WB_b.jpg