ENCAB000BCZ

Antibody against Homo sapiens SKI

Homo sapiens
HEK293T, HepG2, K562
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
HeLa-S3, MCF-7, GM12878, liver
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-9140
Lot ID
F2712
Characterized targets
SKI (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 400-728 mapping at the C-terminus of Ski of human origin
External resources

Characterizations

SKI (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Too many bands, hard to tell which one is correct
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-9140. Molecular mass: 80005 Da
Reviewer comment
Multiple bands, none >50 of total signal in lane. Expected size (~80kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SKI (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-9140 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
No strong band. Also missing expected size and arrow, but even with those, wouldn't be compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford