1. Antibodies
  2. Homo sapiens
  3. RFX1

ENCAB000BCV

Antibody against Homo sapiens RFX1

Homo sapiens
K562, HepG2, MCF-7
characterized to standards
Homo sapiens
GM12878, HeLa-S3, liver
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-10652
Lot ID
E1911
Characterized targets
RFX1 (Homo sapiens)
Host
goat
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Epitope mapping near the C-terminus of RFX1 of human origin
External resources

Characterizations

RFX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-10652. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 3.jpg
RFX1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-10652. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 4.jpg
RFX1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF306YBG. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.963986448 Global enrichment Z-score: 9.4199715 Positional bias Z-score: 13.40611745 Peak rank bias Z-score: 5.689342273 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF654RTP. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.963986448 Global enrichment Z-score: 9.28990809 Positional bias Z-score: 13.19052629 Peak rank bias Z-score: 4.910965851 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-10652.
Reviewer comment
Multiple bands, none >50% of total signal in lane. Expected size (~130kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody sc-10652 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford