ENCAB000BCV
Antibody against Homo sapiens RFX1
Homo sapiens
K562, HepG2, MCF-7
characterized to standards
Homo sapiens
GM12878, HeLa-S3, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-10652
- Lot ID
- E1911
- Characterized targets
- RFX1 (Homo sapiens)
- Host
- goat
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Epitope mapping near the C-terminus of RFX1 of human origin
- External resources
Characterizations
RFX1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-10652. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 3.jpg
RFX1 (Homo sapiens)
MCF-7
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-10652. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 4.jpg
RFX1 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF306YBG. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.963986448 Global enrichment Z-score: 9.4199715 Positional bias Z-score: 13.40611745 Peak rank bias Z-score: 5.689342273 Enrichment rank: 1.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
RFX1 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF654RTP. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.963986448 Global enrichment Z-score: 9.28990809 Positional bias Z-score: 13.19052629 Peak rank bias Z-score: 4.910965851 Enrichment rank: 1.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
RFX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-10652.
- Reviewer comment
- Multiple bands, none >50% of total signal in lane. Expected size (~130kD) not indicated.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RFX1_sc-10652_WB_a.jpg
RFX1 (Homo sapiens)
HeLa-S3
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody sc-10652 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RFX1_sc-10652_WB_b.jpg