ENCAB000BCL

Antibody against Homo sapiens MNT

Homo sapiens
K562, liver, MCF-7, HepG2, HeLa-S3
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-769
Lot ID
C1309
Characterized targets
MNT (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 226-361 of Mnt of human origin
External resources

Characterizations

MNT (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-769. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MNT (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation of MNT from K562 cells using sc-769. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with sc-769. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 62 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
MNT (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody sc-769, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MNT (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-769. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MNT (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-769. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Band shared by IGG control lane, gel is also not clear.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
MNT (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody sc-769. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 62.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
MNT (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using sc-769, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Reviewer comment
Explanation needed for MCM6 and MCM4 ranked higher than MNT
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MNT (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-769.
Reviewer comment
Multiple bands, none >50% of total signal in lane. Expected size (~62kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
MNT (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: MCF7, using the antibody sc-769 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~62 kDa.
Reviewer comment
Multiple prominent bands - may possibly be rescued with a knockdown characterization against the factor.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford