ENCAB000BCI
Antibody against Homo sapiens MLH1
Homo sapiens
GM12878, HepG2, HeLa-S3, MCF-7
partially characterized
Homo sapiens
K562, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-11442
- Lot ID
- C1005
- Characterized targets
- MLH1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Epitope corresponding to amino acids 457-756 mapping at the C-terminus of MLH1 of human origin
- External resources
Characterizations
MLH1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-11442. Molecular mass: 84601 Da
- Reviewer comment
- Multiple bands, none >50% of total signal in lane.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MLH1_sc-11442_WB_a.jpg
MLH1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-11442 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~84 kDa.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MLH1_sc-11442_WB_b.jpg