ENCAB000AZU
Antibody against Homo sapiens SMAD3
Homo sapiens
IMR-90
partially characterized
Homo sapiens
GM12878, K562, HeLa-S3, heart, HepG2, A549
not characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- MA5-14939
- Lot ID
- none
- Characterized targets
- SMAD3 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to residues at the amino terminus of Smad3.
- External resources
Characterizations
SMAD3 (Homo sapiens)
GM12878K562HeLa-S3heartHepG2IMR-90A549
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2, IMR90 and A549 using the antibody MA5-14939. Molecular mass: 48081 Da
- Reviewer comment
- Band is either too faint (lanes 1, 2, 3, 4, and 7) or is not within 20% of expected size (lane 5). Lane 6 is compliant.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMAD3_MA5-14939_WB_a.jpg
SMAD3 (Homo sapiens)
HepG2
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody MA5-14939 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~35 kDa.
- Reviewer comment
- IgG control shares same band
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMAD3_MA5-14939_WB_b.jpg