ENCAB000AZI
Antibody against Homo sapiens ATF6
Homo sapiens
GM12878, K562, HeLa-S3, heart, HepG2, A549
not characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- MA5-16172
- Lot ID
- none
- Characterized targets
- ATF6 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Isotype
- IgG1κ
- Antigen description
- Partial protein containing amino acids 1-273 of human ATF6.
- External resources
Characterizations
ATF6 (Homo sapiens)
GM12878K562HeLa-S3heartHepG2A549
Method: immunoblot
not compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2 and A549 using the antibody MA5-16172. Molecular mass: 74585 Da
- Reviewer comment
- Band is not 50% of overall signal
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ATF6_MA5-16172_WB_a.jpg
ATF6 (Homo sapiens)
HepG2
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody MA5-16172 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Band shared by IgG lane.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ATF6_MA5-16172_WB_b.jpg