1. Antibodies
  2. Homo sapiens
  3. NCOR1

ENCAB000AYZ

Antibody against Homo sapiens NCOR1

Homo sapiens
GM12878, K562, HeLa-S3, heart, HepG2, IMR-90, A549, HeLa
not characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA1-844A
Lot ID
none
Characterized targets
NCOR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR.
Antigen sequence
C P(2427) A P L L S A Q Y E T L S D S D D(2443)
External resources

Characterizations

NCOR1 (Homo sapiens)
GM12878K562HeLa-S3heartHepG2IMR-90A549
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2, IMR90 and A549 using the antibody PA1-844A. Molecular mass: 270210 Da
Reviewer comment
Ladder only goes to 250 kDa, but target is 270 kDa
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
NCOR1 (Homo sapiens)
HeLa
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: Hela, using the antibody PA1-844A .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~270 kDa.
Reviewer comment
Expected size is 270, but ladder stops at 250. Band is also not 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford