ENCAB000AXW

Homo sapiens

any cell type or tissue

characterized to standards with exemption

Method: ChIP-seq comparison

compliant

Caption: This validation relies on the use of antibodies to a histone modification (H3K9me3) in DOHH2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. Please note that the two antibodies originate from two lots of the same vendor antibody part number. The first track shown used an antibody to H3K9me3 previously characterized to Encode standards (PchAb 125-V, ENCAB000APY). The second track shown used an antibody to H3K9me3 (PchAb 634, ENCAB000AXW).
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: H3K9me3 PchAb 634 SAV.pdf

K562

Method: immunoblot

exempt from standards

Caption: This antibody lot did not have a primary completed for it. However, it was used in an experiment where each replicate used a different lot. This characterization is for lot ENCAB000APY. It was used with this lot in experiment ENCSR627TNG.
Submitter comment: This is a request for exemption as the first replicate was done as part of ENCODE2.
Reviewer comment: These experiments with duplicate antibodies were considered valid if they passed replicate concordance.
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: UM1HG009390
Download: H3K9me3_abcamab8898_lot_877391_WB.png
Documents: encode:Histone_Modification_Antibody_Characterization_ENCODE3_Oct2016.pdf