ENCAB000AVZ

Antibody against Homo sapiens HNRNPL

Homo sapiens
HeLa, K562, HepG2
characterized to standards
Status
released
Source (vendor)
Aviva
Product ID
ARP40368_P050
Lot ID
QC9464
Characterized targets
HNRNPL (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
A synthetic peptide directed towards the N terminal region of human HNRNPL
Antigen sequence
AAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYV
Aliases
xiang-dong-fu:HNRNPL
External resources

Characterizations

HNRNPL (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against HNRNPL in HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPL.HNRNPL protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HNRNPL (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against HNRNPL in k562 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPL. HNRNPL protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HNRNPL (Homo sapiens)
HeLa
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation from HeLa whole cell lysate and analized by western blot analysis uisng rabbit polyclonal to HNRNPL. Expected molecular weight: 64 kDa, 50 kDa
Submitted by
Taiki Tsutsui
Lab
Xiang-Dong Fu, UCSD
HNRNPL (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-HNRNPL antibody (lanes under 'anti-HNRNPL'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
HNRNPL (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-HNRNPL antibody (lanes under 'anti-HNRNPL'). Asterisk indicates non-specific band and double asterisk indiecates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
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